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hammerhead ribozyme  (Verlag GmbH)

 
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    Verlag GmbH hammerhead ribozyme
    Hammerhead Ribozyme, supplied by Verlag GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hammerhead+ribozyme/ribozym/pm31553102-94-31-10
    Average 90 stars, based on 1 article reviews
    hammerhead ribozyme - by Bioz Stars, 2026-10
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    Article Title: Riboswitches: ancient and promising genetic regulators.
    Article Snippet: DOI: 10.1002/cbic.200800593 Riboswitches: Ancient and Promising Genetic Regulators Simon Blouin,[a] J r me Mulhbacher,[a] J. Carlos Penedo,[b] and Daniel A. Lafontaine*[a] 400 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim ChemBioChem 2009, 10, 400 – 416

    Article Title: Deoxyribozyme-Based DNA Machines for Cancer Therapy.
    Article Snippet: Deoxyribozymes are single stranded DNA oligonucleotides that catalyze chemical reactions.. First isolated in 1994, RNA-cleaving deoxyribozymes (RCDZ) have been proposed for a number of biotechnological applications.. RCDZ were explored as an alternative to antisense and siRNA gene silencing technologies.



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    Identification of the most efficient Cas12a orthologue and method for crRNA expression. ( a ) Three target sites (T1-T3) in tomato PHYTOENE DESATURASE (SlPDS) were selected. ( b ) Mutation frequencies for three Cas12a orthologues and four methods of crRNA expression at three targets (T1-T3). Note the different y-axis scales for T1, T2, and T3. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies induced by the different orthologues, as determined using Two-Way ANOVA followed by Tukey’s HSD Post-Hoc test. ( c ) For LbCas12a, two additional expression methods using a PolII promoter, and a PolII promoter combined with ribozymes were tested. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies obtained using different crRNA expression methods, as determined using Two-Way ANOVA followed by Tukey’s HSD Post Hoc test.

    Journal: Scientific Reports

    Article Title: Comparison of Cas12a and Cas9-mediated mutagenesis in tomato cells

    doi: 10.1038/s41598-024-55088-4

    Figure Lengend Snippet: Identification of the most efficient Cas12a orthologue and method for crRNA expression. ( a ) Three target sites (T1-T3) in tomato PHYTOENE DESATURASE (SlPDS) were selected. ( b ) Mutation frequencies for three Cas12a orthologues and four methods of crRNA expression at three targets (T1-T3). Note the different y-axis scales for T1, T2, and T3. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies induced by the different orthologues, as determined using Two-Way ANOVA followed by Tukey’s HSD Post-Hoc test. ( c ) For LbCas12a, two additional expression methods using a PolII promoter, and a PolII promoter combined with ribozymes were tested. Error bars indicate standard error (n = 3). Different letters indicate significant differences ( p < 0.05) between mutation frequencies obtained using different crRNA expression methods, as determined using Two-Way ANOVA followed by Tukey’s HSD Post Hoc test.

    Article Snippet: The Hepatitis Delta Virus (HDV) and Hammerhead (HH) ribozymes were amplified from Addgene plasmid #86197, which was a gift from Tang et al ., and similarly cloned to pGEM-T Easy (Promega).

    Techniques: Expressing, Mutagenesis